KMID : 1025520070490060719
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Journal of Animal Science and Technology 2007 Volume.49 No. 6 p.719 ~ p.728
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Analysis of SNPs in Bovine CSRP3, APOBEC2 and Caveolin Gene Family
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Bhuiyan M. S. A.
Yu Seong-Lan Kim Kwan-Suk Yoon Du-Hak Park Eung-Woo Jeon Jin-Tae Lee Jun-Heon
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Abstract
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The cysteine and glycine rich protein 3 (CSRP3), apolipoprotein B mRNA editing enzyme catalytic polypeptide‐like 2(APOBEC2) and caveolin (CAV) gene family(CAV1, CAV2, CAV3) have been reported to play important roles for carcass and meat quality traits in pig, mouse, human and cattle. As an initial step, we investigated SNPs in these 5 genes among eight different cattle breeds. Eighteen primer pairs were designed from bovine sequence data of NCBI database to amplify the partial gene fragments. Sequencing results revealed 9 SNPs in the coding regions of three caveolin genes, 1 SNP in CSRP3 and 3 SNPs in APOBEC2 gene. All the identified SNPs were confirmed by PCR-RFLP. Also, 9 more intronic SNPs were detected in these genes. However, all identified mutations in the coding region do not change amino acid sequence. Allelic distributions were significantly different for 5 SNPs in CAV2, CAV3, CSRP3 and APOBEC2 genes among the eight different breeds. These results gave some clues about the polymorphisms of these genes among the cattle breeds and will be useful for further searches for identifying association between these SNPs and meat quality traits in cattle.
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KEYWORD
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Candidate gene, Single Nucleotide Polymorphism, SNP, Meat quality traits, Allele frequency
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